Application

Research Sub CategoryLive Cell Dye

Live cell fluorescent imaging

Research CategoryCell Imaging

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Lysosomes are membrane-enclosed organelles that contain an array of enzymes capable of breaking down all types of biological material including proteins, nucleic acids, carbohydrates, and lipids. Lysosomes function as the digestive system of the cell, serving both to degrade material taken up from outside the cell and to digest obsolete components of the cell itself.

The BioTracker 560 Orange Lysosome Dye is a live cell fluorescence imaging probe which enhances fluorescence dramatically in acidic environments. This probe can stain acidic organelles such as lysosome, late endosome and granule selectively. Namely, in the condition of pH 5.0, identical to the environment of acidic organelles, fluorescence intensity is 50-fold or more compared to physical pH 7.4. The BioTracker 560 Orange Lysosome Dye shows great photostability against irradiating excitation light. As it emits orange color fluorescent by excitation at 532nm or 514 nm, multicolor imaging is possible by combining with blue fluorescence (CFP, Hoechst, etc.), green fluorescence (GFP, fluorescein, etc.) and near-infrared fluorescence.

Spectral Properties
Absorbance: 535nm
Emission: 560nm

Physical form

Lyophilized

Storage and Stability

Store BioTracker 560 Orange Lysosome Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.